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KMID : 0380219890220010050
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Journal of Biochemistry and Molecular Biology
1989 Volume.22 No. 1 p.50 ~ p.54
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A New Format for Agarose Solid-Phase enzyme Immunoassay
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Kim, Hack Joo
Yang, Jung Ick/Min, Shin Hong/Byun, Si Myung
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Abstract
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A new format for agarose solid-phase enzyme immunosassay (ASEIA) has been developed in which a monoclonal antibody-coupled agarose, packed into a 1-ml disposable plastic syringe, regulates the sample and the enzyme reaction. The assay can be completed in less time at room temperature and in a simpler maner by use of this single-syringe system. The sensitivity of the technique is equivalent to a commercially available enzyme immunoassay kit and was able to detect 1g/L of hepatitis B surface antigen in this study.
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